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通过对菌种、培养基种类及pH、缓冲液种类及pH培养温度、添加葡萄糖和吐温等因素的研究，确定了壮观霉素测定条件，使其测定灵敏度达0．4X10ed。
Through the study of bacteria, culture medium type and pH, buffer type and pH culture temperature, adding glucose and Tween and other factors, the determination conditions of spectinomycin were determined, and its sensitivity was 0.4X10ed.