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目的 研究沙土鼠短暂性全脑缺血后海马CA1区星形细胞反应及亚低温的影响。方法 阻断沙土鼠双侧颈总动脉 2 0分钟造成完全性脑缺血模型。实验动物被随机分为假手术组、缺血再灌流组、亚低温治疗组。免疫组化法用来标记星形胶质细胞。结果 缺血后再灌注 1～ 3天 ,海马CA1区GFAP阳性的星形细胞逐渐增加 ,至再灌注第 7天仍明显高于假手术组。在低温处理的动物 ,星形细胞反应受到显著抑制。结论 在缺血性脑损伤的发展过程中 ,星形胶质细胞反应起了重要作用。亚低温抑制星形胶质细胞反应可能是其神经保护作用的原因
Objective To investigate the effects of hypothermia on the hippocampal CA1 astrocytic response after transient global cerebral ischemia in gerbils. Methods Blocking bilateral common carotid arteries in gerbils for 20 minutes resulted in a model of complete cerebral ischemia. Experimental animals were randomly divided into sham operation group, ischemia-reperfusion group and mild hypothermia treatment group. Immunohistochemistry was used to label astrocytes. Results The number of GFAP positive astrocytes in hippocampal CA1 area increased gradually after reperfusion for 1 to 3 days and remained significantly higher than that of sham operation group on the 7th day after reperfusion. In low temperature treated animals, astrocyte responses were significantly inhibited. Conclusion Astrocyte responses play an important role in the development of ischemic brain injury. Mild hypothermia inhibits astrocyte responses may be responsible for its neuroprotective effects