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目的:探讨CXCR4和CD133在胃癌原发灶中的表达及其对淋巴转移的影响。方法:对50例原发性胃癌原发灶和癌旁胃黏膜组织行免疫组化染色法定位检测CXCR4和CD133蛋白;选用半定量RT-PCR及Western blot法测定CXCR4和CD133 mRNA与蛋白表达量,分析两者的相关性及其与淋巴管浸润和淋巴结转移的关系。结果:CXCR4和CD133分子均定位于肿瘤细胞膜表面,极少数CXCR4位于细胞核内。胃癌组织中CXCR4和CD133表达阳性率及mRNA和蛋白的表达量均明显高于癌旁胃黏膜组织(均P<0.05);CXCR4和CD133 mRNA相对灰度值在淋巴结转移组高于无淋巴结转移组(P=0.011,P=0.038);N1组CXCR4蛋白相对灰度值明显高于N0组(P=0.023),而低于N2+N3组(P=0.008),N1组和N2+N3组CD133蛋白灰度值明显高于N0组(P=0.04,P=0.01),但N1组与N2+N3组之间无明显差异;淋巴管浸润组中的CXCR4和CD133蛋白相对灰度值均高于淋巴管无浸润组(P<0.05);在淋巴转移患者中,CXCR4和CD133蛋白相对灰度值分别与转移淋巴结数(r=0.480,r=0.426)及转移性淋巴结比率(r=0.502,r=0.489)呈正相关。结论:CXCR4和CD133在胃癌原发灶中高表达,两者呈正相关,其联合表达与转移淋巴结比率和转移淋巴结数呈正相关,推测胃癌CD133阳性细胞亚群可能在CXCR4介导下更易导致淋巴管浸润和淋巴结转移。
Objective: To investigate the expression of CXCR4 and CD133 in primary gastric cancer and their effects on lymphatic metastasis. Methods: The expression of CXCR4 and CD133 protein was detected by immunohistochemical staining in 50 cases of primary gastric cancer and adjacent gastric mucosa. The mRNA and protein expressions of CXCR4 and CD133 were detected by semi-quantitative RT-PCR and Western blot , Analyze the correlation between the two and its relationship with lymphatic invasion and lymph node metastasis. Results: Both CXCR4 and CD133 molecules localized on the surface of tumor cell membrane, and a very small number of CXCR4 located in the nucleus. The positive rates of CXCR4 and CD133 and the expressions of both mRNA and protein in gastric cancer tissues were significantly higher than those in the adjacent gastric mucosa tissues (all P <0.05). The relative gray values of CXCR4 and CD133 mRNA in lymph node metastasis group were higher than those without lymph node metastasis group (P = 0.011, P = 0.038). The relative gray value of CXCR4 in N1 group was significantly higher than that in N0 group (P = 0.023), but lower than that in N2 + N3 group Protein gray value was significantly higher than N0 group (P = 0.04, P = 0.01), but there was no significant difference between N1 group and N2 + N3 group; relative gray values of CXCR4 and CD133 protein in lymphatic invasion group were higher than The relative gray values of CXCR4 and CD133 in lymphatic metastasis were significantly correlated with the number of lymph node metastasis (r = 0.480, r = 0.426) and lymph node metastasis (r = 0.502, r = 0.489) was positively correlated. Conclusions: CXCR4 and CD133 are highly expressed in the primary gastric cancer, and there is a positive correlation between the expression of CXCR4 and CD133. The co-expression of CXCR4 and CD133 is positively correlated with the ratio of metastatic lymph nodes and the number of metastatic lymph nodes. It is speculated that the CD133-positive cell subsets of gastric carcinoma may be more easily induced by CXCR4 And lymph node metastasis.