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目的研究调心方有效部位TX0201对杏仁核注射Aβ2535所致类AD模型大鼠氧化损伤及能量代谢的影响。方法通过杏仁核双侧注射Aβ2535片段造成类AD大鼠模型;采用分光光度法检测大脑皮层超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSHPX)活性、活性氧(ROS)的含量以及丙二醛(MDA)含量;原位杂交法检测皮层及海马细胞色素氧化酶Ⅱ型亚基(COⅡ)mRNA的表达。结果AD模型大鼠脑组织SOD、GSHPX活力明显下降,MDA及ROS含量显著上升,同时COIImRNA的表达也明显降低;治疗TX0201组和治疗E2020组大鼠脑组织SOD活力相对于模型组明显提高,MDA和ROS的含量则明显降低,GSHPX活力有升高的趋势;同时治疗TX0201组COIImRNA的表达较模型组有显著提高,而治疗E2020组COIImRNA的表达有上升趋势。结论调心方有效部位TX0201可以明显改善AD大鼠脑内氧化损伤以及能量代谢异常,防治AD的发生与发展。
Objective To investigate the effect of TX0201, an effective component of Tiaoxin Formula, on oxidative damage and energy metabolism in AD rats induced by Aβ2535 injected into the amygdala. METHODS: A rat AD model was induced by bilateral injection of Aβ2535 fragment into the amygdaloid nucleus. Superoxide dismutase (SOD) activity, glutathione peroxidase (GSHPX) activity, and reactive oxygen species (ROS) in cerebral cortex were measured by spectrophotometry. The content of malondialdehyde (MDA) was detected by in situ hybridization and the expression of cytochrome oxidase type II subunit (COII) mRNA in cortex and hippocampus was detected. Results The activity of SOD and GSHPX in brain tissue of AD rats were significantly decreased, and the contents of MDA and ROS were significantly increased. Meanwhile, the expression of CO II mRNA was also significantly decreased. The SOD activity in the brain of rats treated with TX0201 and E2020 was significantly higher than that in the model group. The content of ROS and ROS were significantly decreased, and the GSHPX activity was increased. At the same time, the expression of COII mRNA in the TX0201 group was significantly higher than that in the model group, while the expression of COII mRNA in the E2020 group was increased. Conclusion TX0201, an effective fraction of Tiaoxin Recipe, can significantly improve the oxidative damage and energy metabolism in the brain of AD rats, and prevent the occurrence and development of AD.