• 不 限
  • 期刊论文
  • 硕博论文
  • 会议论文
  • 报 纸
  • 英文论文
  2. 您的位置
  3. 首页
  4. 期刊论文
  5. Development and Preliminary Application of SYBR Green I Real-Time Fluorescence Quantitative PCR Meth

Development and Preliminary Application of SYBR Green I Real-Time Fluorescence Quantitative PCR Meth

来源 :动物与饲料科学：英文版 | 被引量 : 0次 | 上传用户：jayngu
【摘 要】
：
According to VP2 gene sequence of the porcine parvovirus virus strain NADL-2(NC001718) available in GenBank(NC_001718),a pair of specific primer was designed,an
【作 者】
：
SHEN Zhi-qiang WANG Jin-liang 
【机 构】
：
ShandongBinzhouAnimalScience&,VeterinaryMedicineInstitute,ShandongLvduBio-science&,TechnologyC
【出 处】
：
动物与饲料科学：英文版
【发表日期】
：
2009年11期
【关键词】
：
荧光定量PCR Green 猪细小病毒 PCR方法 SYBR 实时定量 检测 特异性引物 Porcine parvovirus virusReal-time f 
下载到本地 , 更方便阅读
下载此文 赞助VIP
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
According to VP2 gene sequence of the porcine parvovirus virus strain NADL-2(NC001718) available in GenBank(NC_001718),a pair of specific primer was designed,and the target fragment of 431 bp was obtained by PCR amplification.The products were ligated wit
其他文献
Comparisons of Protein Composition of Grains at Different Positions in Panicles between Erect and Cu
Under field conditions,three erect panicle type japonica rice cultivars and three curved panicle type japonica rice cultivars were applied as experimental mater
期刊
籽粒蛋白质直立穗型可溶性蛋白含量部位组成比粳稻品种蛋白质成分稻穗Japonica rice Panicle type Grain positio
Application of Real-time Fluorescent Quantitative PCR in Studies on Plants
Real-time fluorescent quantitative PCR is a method for quantitative analysis of gene expression developed in recent years, which has been widely used in various
期刊
实时荧光定量PCR技术植物应用基因表达科学研究临床诊断定量分析基因鉴定Real-time fluorescent quantitative PC
新时期地方气象部门防雷工作的重点及发展思路
结合重庆市巴南区气象局开展防雷减灾管理工作现状,阐述了新时期地方气象部门防雷工作重点,并分析了当前地方防雷工作中存在的问题,提出了防雷未来发展思路,以供有关部门参考
期刊
新时期地方气象部门防雷发展思路
桂林市2~3岁儿童行为问题及干预对策
目的:了解桂林市2~3岁儿童行为问题的发生模式,为进一步干预提供依据。方法:用Achenbach(CBCL)2~3岁儿童行为量表(家长问卷)调查2~3岁儿童2个月内的行为状况。结果:我市2~3岁
期刊
行为问题干预儿童
生鲜农产品冷链物流的标准化问题研究
冷链物流是作为现代物流体系中需求较大、专业性最强的行业物流,其技术发展和管理规范也越来越受人关注。对于冷链物流标准的制定,是冷链物流服务行业发展的需要,也是保证冷
期刊
生鲜农产品冷链物流标准化
Chromosomal Transmission in F2 BC1 Hybrid Progenies between Sugarcane and Erianthus fulvus
[Objective]This study aimed to provide a scientific basis for breeding new sugarcane varieties with hybrid progenies of Erianthus fulvus and modifying existing
期刊
体细胞染色体数目杂交后代甘蔗品种BC1F2斑茅传输材料制备Sugarcane Erianthusfulvus Hybrid Chromosoma
The Influence of Online Reviews on Consumers＇ Purchase Decision -- An Empirical Study
期刊
消费者行为决策机制评论在线学术研究营销Online Reviews Trust Consumer Purchasing Decision Em
Cutting Rapid Propagation Technology for Antigonon leptopus in Tropical Areas
The shoots with good functional leaves were selected,from which cutting slips with the length of about 20 cm were cut,and 2 nodes were left for the ones with lo
期刊
快速繁殖技术热带地区珊瑚藤扦插切割长度切割深度景观效果发展前景Antigonon leptopus Cutting Rapid propagat
Establishment and Optimization of ISSR Reaction System for Elymus sibiricus L.
[Objective] This study aimed to establish and optimize the ISSR-PCR reaction system and amplification process for Elymus sibiricus L., to provide scientific bas
期刊
ISSR反应体系老芒麦优化PCR反应体系DNA聚合酶dNTP浓度最佳反应体系ISSR分析Elyrnus sibiricus L. ISSR O
注册会计师行业发展存在的问题分析
注册会计师行业的英文缩写是CPA,有着"经济警察"的称号,督促着各企业公司的健康发展,特别是担任着独立审计的重任。改革开放后,我国的注册会计师行业为我国的经济建设做出了
期刊
注册会计师行业发展问题分析