Establishment and Optimization of ISSR Reaction System for Elymus sibiricus L.

• 作 者: Guodong ZHOU Zhiyong LI Shun H
• 机 构: GrasslandResearchInstitute,GraduateSchool,InnerMongoliaAgriculturalUniversity
• 出 处: 农业生物技术：英文版
• 发表日期: 2012年5期
• 关键词: ISSR反应体系 老芒麦 优化 PCR反应体系 DNA聚合酶 dNTP浓度 最佳反应体系 ISSR分析 Elyrnus sibiricus L. ISSR O
• 基金项目: Supported by Project of Collection, Cataloguing and Utilization of Perennial Forage Grass Germplasm Resources （ NB2012-2130135-33 ） , Conservation Project for Forage Grass Germplasm Resources.

[Objective] This study aimed to establish and optimize the ISSR-PCR reaction system and amplification process for Elymus sibiricus L., to provide scientific basis for exploring the genetic diversity of E. sibiricus germplasm resources.[Method] Orthogonal