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在印度南部的臭椿树上有两种引人瞩目的鳞翅目害虫臭椿皮蛾和乔椿巢蛾。1983年9月至12月,此地蔓延一种疫病,使很多臭椿皮蛾致死。死虫悬挂于树叶上。从感病幼虫的肠道内含物和渗出物中,相继发现一种杆状细菌,为确定此种细菌的致病性,进行了试验研究。将在营养琼脂培养基上生长48—72小时的细菌培养物,悬浮在无菌蒸馏水中,制备成悬浮液。用1×10~(10)CFU/ml 活细胞数在420毫微米处给出10D 值的吸收率来标定该悬浮液。接种试验材料为不同龄期的健康幼虫。用一个经灭菌的无菌喷雾器,把细菌悬浮液喷在臭椿叶上或用刷子把菌液刷在叶片上,然后将10条幼
There are two striking Lepidoptera pests, Crab Abbey and Jiao Chun nest, on the tree of Ailanthus in southern India. From September to December 1983, a disease was spread here that killed many Crabgrass moths. Dead insects hang on the leaves. From the gut contents and exudates of susceptible larvae, a bacterium was found one after another. To determine the pathogenicity of this bacterium, experimental studies were carried out. Bacterial cultures grown on nutrient agar medium for 48-72 hours are suspended in sterile distilled water to make a suspension. The suspension was calibrated by giving an absorbance of 10D at 420 nm with 1 x 10 ~ (10) CFU / ml viable cells. Inoculation test materials are healthy larvae of different ages. Using a sterilized sterile sprayer, spray the bacterial suspension on the stinkbug leaves or brush the brushes on the leaves and leave the 10