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: [目的 ]选出一种简便、可靠和易推广的实验方法进行人群麻疹免疫状态监测。 [方法 ]对同一批血清标本用酶联免疫吸附试验 (EL ISA)与血凝抑制试验 (HI)两种方法进行检测。 [结果 ]两种方法结果呈高度正相关 (r=0 .70 ,P<0 .0 0 1) ,检出符合率为 86 .9% ,检出阳性率无显著性差别 (χ2 =0 .5 3,P>0 .30 )。 HI法检出结果为阴性的标本 ,用EL ISA法检测 ,有 92 .0 %的标本抗体滴度≤ 1∶ 2 0 0 ;反之 ,EL ISA法检出结果为阴性的标本 ,用 HI法检测 ,有 90 .3%的血清抗体滴度≤ 1∶ 4。两种方法的一致性很好。 [结论 ]EL ISA法易操作 ,结果可靠 ,有推广使用价值。
: [Objective] To select a simple, reliable and easy to popularize experimental method for measles immune status monitoring. [Method] The same batch of serum samples were detected by enzyme-linked immunosorbent assay (ELISA) and hemagglutination inhibition test (HI). [Results] The results of the two methods were highly positive (r = 0.70, P <0.001), and the coincidence rate was 86.9%. There was no significant difference between the two methods (χ2 = 0. 5 3, P> 0 .30). HI method to detect the negative results of the sample, using EL ISA test, 92.0% of the sample antibody titer ≤ 1:20; the contrary, EL ISA negative results were detected by HI method , 90.3% of the serum antibody titers ≤ 1: 4. The consistency of the two methods is good. [Conclusion] The EL ISA method is easy to operate, the result is reliable and has the value of popularization and use.