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已经表明成人孤立喉乳头状瘤(ASLP)常经1～2次手术可治愈,这与多发成人和青少年型乳头状瘤病程长相反,ASLP的组织学检查有凹型细胞,提示HPV感染。但是ASLP是否存在HPV_(6,11)尚有争论。而多发乳头状瘤已多次显示存在HPV。作者对16例ASLP_20块活组织及一例过度角化乳头状瘤做组织学检查,并用大肠杆菌感染HPV后自制DNA探针,经磺化处理后对组织切片进行原位杂交。结果组织学示皆为良性鳞状细胞乳头状瘤,无增生不良。大部分病例表面角化缺如,偶见角化不全。组织中皆可见凹型细胞,提示HPV感染。过度角化乳头状瘤表面显著角化,未见真正凹型细胞。原位杂
It has been shown that adult solitary laryngeal papilloma (ASLP) is curable with 1 to 2 surgeries, which is contrary to the long duration of multiple adult and adolescent papilloma. The histological examination of ASLP has concave cells, suggesting HPV infection. However, there is still controversy over whether there is HPV_(6,11) in ASLP. Multiple HPVs have repeatedly shown the presence of HPV. The authors performed histological examinations of 16 cases of ASLP_20 biopsy and one case of hyperkeratotic papillomas, and infected HPV with E. coli. After self-made DNA probes, the tissue sections were subjected to in situ hybridization after sulfonation. Results Histopathology showed benign squamous cell papilloma without dysplasia. In most cases, surface keratinization is absent and occasionally parakeratosis is incomplete. Concave cells are visible in the tissue, suggesting HPV infection. The hyperkeratotic papilloma was significantly keratinized on the surface and no true concave cells were seen. Miscellaneous