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作者应用碱性磷酸酶-抗碱性磷酸酶(APAAP)和生物素-亲和素(ABC)双标记技术对肾病综合征出血热(HFRS)患者不同病期外周血单个核细胞(PBMC)中的淋巴细胞群和亚群进行了表型分析。同时,应用ELISA夹心法测定不同时期HFRS患者和正常人血浆(清)中可溶性白细胞介素-2受体(sIL-2R)的水平。结果表明,急性期(包括发热期、休克期与少尿期)HFRS患者PBMC中T淋巴细胞(CD_3)减少,CD_4:CD_8细胞比率下降,B细胞增多。但急性期HFRS患者PBMC中激活抗原(CD_(25)、TLiSA1、CD_(71)和Ia)阳性的淋巴
The authors used alkaline phosphatase-resistant alkaline phosphatase (APAAP) and biotin-avidin (ABC) dual-labeling technique to detect peripheral blood mononuclear cells (PBMCs) in patients with nephrotic syndrome hemorrhagic fever (HFRS) The lymphocyte population and subpopulations were phenotypically analyzed. Meanwhile, the level of soluble interleukin-2 receptor (sIL-2R) in plasma (serum) of HFRS patients and normal persons at different periods was measured by ELISA sandwich method. The results showed that the number of T lymphocytes (CD_3), the percentage of CD_4: CD_8 cells and the number of B cells in PBMC of HFRS patients in acute stage (including fever period, shock period and oliguria period) decreased. However, the positive lymphocytes of activated PBMCs (CD_ (25), TLiSA1, CD_ (71) and Ia) in PBMC of acute stage HFRS patients