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应用抗p53单克隆抗体及LSAB免疫组织化学染色法检测喉鳞癌、喉癌旁组织及声带息肉p53蛋白的表达。结果显示:25例(57％)喉鳞癌呈阳性反应,4例(20％)距肿瘤边缘<0.5cm的喉癌旁组织呈弱阳性反应,20例距肿瘤边缘>2cm的喉癌旁组织及16例声带息肉均为阴性。p53表达与喉癌分型、分期及淋巴结转移不相关,而与病理分化程度密切相关(P<0.01)。表明,抑癌基因p53的突变是肿瘤增生异常活跃的因素之一,在喉癌的发生中可能是一种比较常见的基因改变,并与喉癌的恶性程度有关。
The expression of p53 protein in laryngeal squamous cell carcinoma, adjacent laryngeal tissues and vocal cord polyps was detected by anti-p53 monoclonal antibody and LSAB immunohistochemical staining. The results showed that 25 cases (57%) of laryngeal squamous cell carcinoma positive reaction, 4 cases (20%) from the edge of the tumor <0.5cm laryngeal cancer tissue weak positive reaction, 20 cases away from the edge of the tumor> 2cm laryngeal cancer tissue And 16 cases of vocal cord polyps were negative. The expression of p53 was not correlated with the type, stage and lymph node metastasis of laryngeal carcinoma, but closely correlated with the degree of pathological differentiation (P <0.01). It is indicated that the mutation of tumor suppressor gene p53 is one of the active factors of abnormal tumor proliferation. It may be a common genetic change in the occurrence of laryngeal cancer and is related to the malignancy of laryngeal cancer.