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目的:观察先天性巨结肠(HD)不同节段肠壁层粘连蛋白(LN)的表达,探讨LN与HD发病的关系。方法:收集30例手术切除HD标本,分别行LN免疫组化研究和实时荧光定量PCR,观察其分布情况,并行统计学处理。结果:LN染色面积及染色强度从狭窄段到正常段依次减弱,差异有统计学意义(P<0.05);LN基因在狭窄段的表达是正常段的2.15倍,其表达水平狭窄段>移行段>正常段。结论:HD中LN的分布出现异常,是HD发病的重要原因。
Objective: To observe the expression of LN in different segments of Hirschsprung ’s disease (HD) and to explore the relationship between LN and HD. Methods: Thirty patients undergoing surgical resection were enrolled in this study. LN immunohistochemistry and real-time fluorescence quantitative PCR were performed respectively. The distribution of HD was observed and statistically analyzed. Results: The LN staining area and staining intensity decreased in the order from the stenosis to the normal, with significant difference (P <0.05). The LN gene expression in the stenosis was 2.15 times of that in the normal > Normal section. Conclusion: The abnormal distribution of LN in HD is an important cause of HD.