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目的 观察干扰素 (IFN a)和肿瘤坏死因子 (TNF a)单独应用或与化疗药物联合作用于肿瘤细胞的凋亡诱导作用。方法 形态学观察和流式细胞仪检测细胞因子对培养的人早幼粒白血病细胞(HL 6 0 )、小鼠淋巴细胞性白血病细胞 (L 12 10 )和小鼠肝癌细胞 (H2 2 )的凋亡诱导作用。结果 用光学显微镜、荧光显微镜和电子显微镜均观察到细胞因子诱导肿瘤细胞凋亡的典型形态学改变 ,流式细胞仪检测结果表明 ,干扰素与阿糖胞苷 (Ara c)合用或肿瘤坏死因子与阿糖胞苷合用可以显著提高肿瘤细胞凋亡率 ,并可明显抑制肿瘤细胞DNA的合成 ,使S期和G2 M期的细胞显著减少 ,而G0 G1 期细胞增多 ,因而使增殖指数 (PI)显著降低 (P <0 .0 1)。结论 某些细胞因子与化疗药联合使用可以起到增强抗肿瘤的疗效作用。
Objective To observe the apoptosis-inducing effect of interferon (IFN a) and tumor necrosis factor (TNF a) alone or in combination with chemotherapeutic drugs for tumor cells. Methods Morphological observation and flow cytometry were used to detect the apoptosis of cultured human promyelocytic leukemia cells (HL 60), mouse lymphocytic leukemia cells (L 12 10) and mouse hepatoma cells (H 2 2). Induced by death. Results The typical morphological changes of apoptosis induced by cytokines were observed by light microscopy, fluorescence microscopy, and electron microscopy. The results of flow cytometry showed that interferon combined with cytarabine (Ara c) or tumor necrosis factor In combination with cytarabine, the apoptosis rate of tumor cells can be significantly increased, and the synthesis of DNA in tumor cells can be significantly inhibited, so that the cells in S phase and G2 M phase are significantly reduced, while the cells in G0 G1 phase are increased, thus making the proliferation index (PI). Significantly lower (P < 0.01). Conclusion The use of certain cytokines in combination with chemotherapeutic drugs can enhance antitumor efficacy.