论文部分内容阅读
在附加不同浓度ＮＡＡ，６—ＢＡ的ＭＳ培养基上对杜仲的下胚轴和子叶进行愈伤组织的诱导并获得两类不同的愈伤组织，一类是白色的结构松散的非胚性愈伤组织，虽然能继代培养，但在附加较高浓度的６─ＢＡ的ＭＳ培养基上都不能再生植株；另一类是黄绿色的结构紧密的有颗粒状突起的胚性愈伤组织．在附加适量的６—ＢＡ的ＭＳ培养基上能较容易再生小植株。实验表明，杜仲的下胚轴和子叶在附加１．０ｍｇ／ＬＮＡＡ和１．０ｍｇ／Ｌ６—ＢＡ的ＭＳ培养基上能１００％诱导成胚性愈伤组织；胚性愈伤组织在附加０．５ｍｇ／ＬＮＡＡ和２．５ｍｇ／Ｌ６—ＢＡ的ＭＳ培养基上培养，植株再生频率高达９２％；小植株转移到附加１．５ｍｇ／Ｌ的ＩＢＡ的１／２ＭＳ培养基上，１５ｄ左右长出较粗的根。
The callus induction on the hypocotyls and cotyledons of Eucommia ulmoides subtilis was induced on MS medium supplemented with different concentrations of NAA and 6-BA, and two types of callus were obtained, one is white and the other is non-embryogenic Injured tissue, although subcultured, failed to regenerate plants on MS medium supplemented with higher concentrations of 6─BA; the other was embryonic callus with yellowish green structure and granular projections. Plantlets can be regenerated more easily with an appropriate amount of 6-BA in MS medium. Experiments show that the hypocotyls and cotyledons of Eucommia ulmoides callus can be induced into embryogenic callus 100% on MS medium supplemented with 1.0mg / L NAA and 1.0mg / L 6-BA; 5mg / L NAA and 2.5mg / L 6-BA MS medium, the frequency of plant regeneration up to 92%; plantlet transferred to additional 1.5mg / L IBA 1 / 2MS medium, grow about 15d more Rough roots.