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目的探讨碘缺乏和碘过量对子代小鼠海马CA3区神经元的影响。方法断乳后1个月Balb/c小鼠,雌雄各半,随机分为5组:低碘组(LI),适碘组(NI),5倍碘组(5HI),10倍碘组(10HI),50倍碘组(50HI),给予不同浓度的碘化钾碘水,饲养3个月后雌雄合笼,取40日龄子代小鼠脑组织,应用免疫组织化学技术观察海马CA3区神经元,并进行形态计量学分析。结果与NI组相比,5HI组、10HI组NSE阳性神经元面数密度及平均光密度无明显变化,差异无统计学意义;LI组、50HI组面数密度及平均光密度有不同程度的下降,且有统计学意义。结论脑发育临界期碘缺乏及严重碘过量会影响子代小鼠海马神经元的发育,且碘缺乏的影响较碘过量严重。
Objective To investigate the effects of iodine deficiency and iodine excess on neurons in hippocampal CA3 region of offspring mice. Methods One month after weaning, Balb / c mice were randomly divided into 5 groups: low iodine group (NI), iodine group (5HI), iodine group (10HI) 10HI), 50 times iodine group (50HI), given different concentrations of potassium iodide water, feeding 3 months after the male and female cage, take the 40-day-old offspring mouse brain tissue, the use of immunohistochemistry to observe the hippocampal CA3 neurons , And morphometry analysis. Results Compared with NI group, the number density and average optical density of NSE positive neurons in 5HI group and 10HI group had no significant difference, and the difference was not statistically significant. The number density and average optical density of NSE positive neurons in LI group and 50HI group decreased to some extent , And has statistical significance. Conclusions Iodine deficiency and severe iodine excess in critical stage of brain development may affect the development of hippocampal neurons in offspring mice, and the effect of iodine deficiency is more serious than iodine excess.