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为研究丙酸睾酮对神经脱细胞支架桥接修复犬坐骨神经缺损的作用,选杂种犬6只,手术制备坐骨神经3 cm缺损模型,并用同种异体神经脱细胞支架桥接修复,将修复犬随机均分为试验组和对照组。试验组在犬修复肢小腿外侧注射丙酸睾酮;对照组在犬相应部位注射等量生理盐水。术后4个月,行肌电图检测、腓肠肌湿重比率、腓肠肌和修复神经的组织形态学观察。结果显示,试验组和对照组腓肠肌湿重比率分别是(77.73±2.72)%、(64.59±3.98)%,神经传导速度分别是(21.23±2.23)m/s、(15.7±1.02)m/s,波幅分别是(1.64±0.89)mv、(1.28±0.82)mv。两组间湿重比率、传导速度、波幅均差异显著(P<0.05)。试验组中的再生神经纤维数量多于对照组,板层结构清晰完整且优于对照组。结果表明,丙酸睾酮对同种异体神经脱细胞支架桥接修复犬坐骨神经缺损和生理功能恢复均具有促进作用。
In order to study the effect of testosterone propionate bridging repaired sciatic nerve defects of canine acellular scaffolds, 6 dogs were selected and sciatic nerve 3 cm defect models were prepared surgically and repaired by allogeneic decellularized scaffolds. The dogs were randomly divided into Experimental group and control group. The test group was injected with testosterone propionate on the lateral side of the leg of the dog, while the control group was injected with the same amount of saline on the corresponding part of the dog. At 4 months after operation, EMG test, gastrocnemius wet weight ratio, gastrocnemius muscle and nerve repair were observed. The results showed that the gastrocnemius wet weight ratio of the experimental group and the control group were (77.73 ± 2.72)% and (64.59 ± 3.98)%, respectively. The nerve conduction velocities were (21.23 ± 2.23) m / s and (15.7 ± 1.02) m / , The amplitude was (1.64 ± 0.89) mv, (1.28 ± 0.82) mv. The wet weight ratio, conduction velocity and amplitude were significantly different between the two groups (P <0.05). The number of regenerated nerve fibers in the experimental group was more than that of the control group, the lamellar structure was clear and complete and better than the control group. The results showed that testosterone propionate promoted the repair of sciatic nerve defects and recovery of physiological function in allogeneic decellularized scaffolds.