论文部分内容阅读
目的用显微硬度测量法研究酪蛋白磷酸肽-无定形磷酸钙(casein phosphopeptide-amorphous calcium phos-phate,CPP-ACP)对碳酸饮料酸蚀后的牙釉质的再矿化作用。方法收集临床拔除的第三磨牙共15颗,制成牙釉质样本30个,完全随机分为:去离子水组(阴性对照组)、CPP-ACP组(实验组)、氟保护漆组(阳性对照组),每组10个样本。各组样本分别用可口可乐酸蚀,7次/d,每次2 min,8 h内完成,共7 d,然后分别进行再矿化处理。去离子水组置于去离子水中,不做其他处理;CPP-ACP组和氟保护漆组分别用CPP-ACP和氟保护漆处理。分别在酸蚀前、酸蚀后和再矿化后用维氏显微硬度计测定釉质表面硬度并进行扫描电镜观察。结果经可口可乐酸蚀后的牙釉质表面硬度值较酸蚀前有明显下降(P<0.05);经再矿化处理后,CPP-ACP组和氟保护漆组牙釉质表面硬度值与去离子水组相比均有统计学差异(P<0.05);CPP-ACP组和氟保护漆组之间的差异有统计学意义(P<0.05)。扫描电镜观察结果显示:CPP-ACP组和氟保护漆组经过再矿化处理后,脱矿釉质表面有矿物质沉积。结论 CPP-ACP能促进钙盐沉积于脱矿釉质表面而提高酸蚀牙釉质的显微硬度,显著促进酸蚀釉质再矿化。
Objective To study the remineralization effect of casein phosphopeptide-amorphous calcium phos-phate (CPP-ACP) on enamel after acid-etching of carbonated beverages by microhardness measurement. Methods A total of 15 third molars were collected and 30 enamel samples were collected. They were randomly divided into three groups: deionized water group (negative control group), CPP-ACP group (experimental group) and fluoride protective paint group Control group) with 10 samples in each group. The samples of each group were respectively treated with Coca-Cola acid etching for 7 times / d for 2 min and 8 h respectively, for a total of 7 days, then were remineralized respectively. Deionized water was placed in deionized water, without any other treatment; CPP-ACP group and fluoride protective paint group were treated with CPP-ACP and fluoride protective paint. The surface hardness of the enamel was measured before and after acid etching and remineralization, respectively. The surface hardness of the enamel was measured by scanning electron microscopy. Results The hardness of enamel treated with Coca-Cola was significantly lower than that before acid etching (P <0.05). After remineralization, the surface enamel hardness of CPP-ACP group and fluoride-protective lacquer group were similar to those of deionized water (P <0.05). The difference between CPP-ACP group and fluoride-protective paint group was statistically significant (P <0.05). Scanning electron microscopy results show that: CPP-ACP group and fluoride protective paint group after remineralization, demineralization of enamel surface mineral deposits. Conclusion CPP-ACP can promote the deposition of calcium salt on the surface of demineralized enamel and improve the microhardness of acid-etched enamel, and promote the remineralization of acid-enamel significantly.