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目的:研究红霉素及左氧沙星联合应用时抗解脲脲原体临床株最小抑菌浓度(MIC)的影响。方法:用微量液体倍比稀释法及平板棋盘法分别测定单药及联合时对解脲脲原体(Uu)的MIC,并进行FIC计算及统计分析。设定红霉素及左氧沙星的MIC≥8mg.L-1为耐药。结果:共51株标本。联合应用后,红霉素耐药株MIC降低(P<0.05),而敏感株升高(P<0.05);而左氧沙星无论是耐药株还是敏感株的MIC均为降低(P<0.05),耐药株更明显。对红霉素及左氧沙星同时敏感的菌株,联合后红霉素的MIC升高(P<0.05)。在红霉素耐药株及左氧沙星耐药株中,联合后结果为拮抗的比敏感株明显减少。结论:左氧沙星与红霉素联合,有助于降低左氧沙星的MIC及红霉素耐药株对红霉素的MIC,逆转红霉素耐药株对红霉素的耐药性,但也可能增强红霉素敏感株对红霉素的耐药性。
Objective: To study the effect of combined use of erythromycin and levofloxacin on the minimum inhibitory concentration (MIC) of clinical isolates of Ureaplasma urealyticum. Methods: MICs of Uu were determined by microdialysis of liquid multiples and plate chessboard method respectively. FIC and FIC were calculated. Set erythromycin and levofloxacin MIC ≥ 8mg.L-1 is resistant. Results: A total of 51 specimens. After combined application, MICs of erythromycin-resistant strains decreased (P <0.05) and the susceptible strains increased (P <0.05), while MIC of levofloxacin-resistant and susceptible strains decreased (P < 0.05), resistant strains more obvious. Erythromycin and levofloxacin simultaneous sensitive strains of erythromycin combined MIC (P lt; 0.05). In the erythromycin-resistant and levofloxacin-resistant strains, the ratio-sensitive strains with antagonistic results were significantly reduced. Conclusion: The combination of levofloxacin and erythromycin can reduce the MIC of levofloxacin and erythromycin MIC of erythromycin resistant strains, and reverse the erythromycin resistance of erythromycin resistant strains However, it may also increase the erythromycin resistance of erythromycin sensitive strains.