人工合成Bt基因对棉花的遗传转化

• 作 者: 张震林 倪万潮 郭三堆 束春娥 张保龙 徐英俊
• 机 构: 江苏省农业科学院经济作物研究所,中国农业科学院生物技术中心,江苏省农业科学院植物保护研究所
• 出 处: 江苏农业学报
• 发表日期: 1998年03期
• 关键词: Bt基因 人工合成 中棉所 杀虫蛋白 高效表达载体 遗传转化 翻译能力 抗虫性 基因表达载体 抗虫基因

采用子房注射法，将构建在植物高效表达载体上的全序人工合成的Ｂｔ杀虫基因ＧＦＭＣｒｙＩＡ转入棉花品种（系）“中棉所１９”、“华农９１６”、“５４１”和“３５１７”中，获得对棉铃虫幼虫致死率在８０％以上的植株２９株。用ＰＣＲ法检测，结果表明这些植株均呈阳性。在这２９株阳性植株中，对棉铃虫致死率为１００％，且自身只有轻微伤害的植株有２６株。这说明Ｂｔ基因经改造，增加其提高转录与翻译能力的表达调控元件后，对棉铃虫的毒杀作用明显提高。

The full-sequence synthetic Bt insecticidal gene GFMCryIA, which was constructed on the high efficient plant expression vector, was transferred into cotton cultivar “Zhongmiansuo 19”, “Hua Nong 916”, “541” and “3517 ”, Obtaining 29 plants which kill 80% of the bollworm larvae. By PCR method, the results showed that these plants were positive. Among the 29 positive plants, there were 26 plants that gave 100% lethality to the cotton bollworm and only a few minor injuries to themselves. This shows that the Bt gene was modified to increase its ability to improve transcription and translation of regulatory elements of expression, the toxicity of cotton bollworm significantly increased.