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目的探讨调节正常T细胞表达和分泌活性因子(regulated on activation,normal T cell expressed and secreted,RANTES)基因启动子区-28C/G单核苷酸的多态性与广东籍汉族患者子宫内膜异位症的关系。方法应用聚合酶链反应-限制性酶切片段长度多态性技术(PCR-RFLP)并进行基因测序的方法检测广东籍汉族子宫内膜异位症患者59例(内异症组),非子宫内膜异位症患者49例(对照组),比较分析各组间基因型频率和等位基因频率。结果 RANTES基因启动子区-28C/C基因型在子宫内膜异症组及对照组分布频率分别为81.36%、81.63%,C/G基因型分布频率分别为18.64%、18.37%;两组间的基因型分布频率比较差异无显著性(P>0.05)。RANTES基因启动子区-28位点C等位基因型在内异症组及对照组中的分布频率分别为90.68%、90.82%,G等位基因型分布频率分别为9.32%、9.18%,两组间等位基因型频率比较差异无统计学意义(P>0.05)。结论在广东籍汉族妇女中,RANTES基因启动子区-28C/G单核苷酸多态性与子宫内膜异位症遗传易感性可能无关联。
Objective To investigate the relationship between the polymorphisms of -28C / G single nucleotide that regulate the promoter region of normal T cell expressed and secreted (RANTES) gene and normal endometrium in Guangdong Han patients Symptoms of the relationship. Methods 59 patients with endometriosis (endometriosis group) and non-uterus from Han nationality in Guangdong province were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and gene sequencing method In 49 patients with endometriosis (control group), genotype frequencies and allele frequencies were compared between groups. Results The distribution frequencies of -28C / C genotype of RANTES gene in endometriosis group and control group were 81.36% and 81.63%, respectively. The frequencies of C / G genotype were 18.64% and 18.37% There was no significant difference in genotype frequencies (P> 0.05). The frequencies of C allele at -28 locus of RANTES gene in endometriosis group and control group were 90.68% and 90.82%, respectively. The distribution frequency of G allele was 9.32% and 9.18% There was no significant difference in allele frequencies between groups (P> 0.05). Conclusion There is no correlation between -28C / G SNP in RANTES promoter region and susceptibility to endometriosis in Han women of Guangdong ethnicity.