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目的 研究在不同放射性浓度下, 5 125I 2′ 脱氧尿嘧啶核苷(125I UdR)对大鼠C6 神经胶质瘤细胞的杀伤效应,并初步探讨其发生机制。方法 在大鼠C6神经胶质瘤细胞的培养液中,加入不同放射性浓度 125I UdR培养 24h后,通过细胞形态学变化、细胞存活实验及流式细胞仪检测细胞凋亡率进行观察。结果 在加入 74MBq/L放射性浓度的 125IUdR培养 24h后,细胞形态上表现出皱缩、变圆、脱落现象,贴壁细胞数目减少。不同放射性浓度的细胞存活分数 (SF)随着浓度升高明显下降,同一浓度下与对照组相比较,存在统计学意义。同时流式细胞仪 (FCM)检测随着 125I UdR的升高,细胞凋亡率增加, 24h细胞凋亡率最高接近 20%。结论 125I UdR对大鼠C6 神经胶质瘤细胞具有明显的杀伤效应,并随着浓度的提高而增强,其发生机制可能是通过诱导细胞凋亡而产生。
Objective To investigate the killing effect of 5 125I 2 ’deoxyuridine (UIR) on C6 glioma cells under different radioactive concentrations and to explore its possible mechanism. Methods The C6 glioma cells were cultured in different concentrations of radioactive 125I UdR for 24 hours. Morphological changes, cell survival and flow cytometry were used to observe the apoptosis rate. Results After cultured with 125IUdR of 74 MBq / L for 24 h, the cell morphology showed shrinkage, rounding and shedding, and the number of adherent cells decreased. The cell viability (SF) of different radioactive concentrations decreased significantly with the increase of concentration, and there was statistical significance compared with the control group at the same concentration. Flow cytometry (FCM) detection with the increase of 125I UdR, the rate of apoptosis increased, the highest rate of apoptosis of 24h close to 20%. Conclusion 125I UdR has obvious cytotoxicity on rat C6 glioma cells, which is enhanced with the increase of concentration. The mechanism may be induced by apoptosis.