论文部分内容阅读
目的 :探讨血凝素样氧化低密度脂蛋白受体 (LOX1)在氧化低密度脂蛋白 (ox -LDL)致内皮细胞损伤中的作用。方法 :采用倒置相差显微镜观察细胞形态的改变 ;发色底物法检测内皮细胞培养液中的组织纤溶酶原激活物 (t -PA)和纤溶酶原激活物抑制剂 (PAI - 1)的活性 ;反转录聚合酶链反应 (RT -PCR)检测LOX1mRNA表达水平。结果 :单纯加入ox -LDL ,内皮细胞出现胞体收缩 ,细胞膜破坏等明显的损伤性改变 ,培养液中PAI - 1活性较对照组高 3倍 (P <0 .0 5 ) ,LOX1mRNA水平表达增高 ;而当培养液中同时加有LOX1抑制剂polyinosinicacid时 ,内皮细胞形态损伤不明显 ,培养液中PAI - 1活性低约 2 .6倍 (P <0 .0 5 ) ,同时LOX1mRNA水平降低。结论 :LOX1介导了ox -LDL对内皮细胞的损伤 ,可能在血管损伤性疾病的发生中起重要作用
Objective: To investigate the role of hemagglutinin-like oxidized low density lipoprotein receptor (LOX1) in endothelial cell injury induced by ox-LDL. Methods: The changes of cell morphology were observed by inverted phase contrast microscope. Tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI - 1) in endothelial cell culture medium were detected by chromogenic substrate method. The activity of LOX1 mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: In ox - LDL group alone, the injury of cell bodies and cell membrane were obvious. The activity of PAI - 1 in culture medium was 3 times higher than that in control group (P <0.05), and the expression of LOX1 mRNA increased. When polyinosinic acid was added into culture medium simultaneously, the morphological damage of endothelial cells was not obvious. The activity of PAI - 1 in culture medium was about 2.6 times lower (P <0.05), and the level of LOX1 mRNA was also decreased. Conclusion: LOX1 mediates the damage of endothelial cells by ox-LDL and may play an important role in the development of vascular injury