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目的:观察红景Ⅰ方对低氧状态下大鼠阴茎海绵体平滑肌细胞表型转化的影响。方法:将120只SD大鼠随机分为常氧组、低氧模型组、前列腺E1组、红景Ⅰ号方低剂量组、红景Ⅰ号方中剂量组、红景Ⅰ号方高剂量组,每组20只,分别测定各组大鼠勃起情况、RT-PCR测定α-actin、OPN的相对表达量。结果:造模6周后,与常氧组比较,模型组勃起次数明显降低(P<0.01),低氧实验动物模型建立成功。与常氧组比较,低氧模型组的α-actin含量显著降低(P<0.01),OPN含量明显升高(P<0.01)。与低氧模型组比较,高中低剂量的红景Ⅰ号组α-actin含量上升(P<0.05),其中高中剂量组含量上升明显(P<0.01);OPN含量降低(P<0.05),其中高中剂量组含量下降明显(P<0.01)。结论:红景Ⅰ号方有抑制海绵体平滑肌细胞表型转化的作用。
Objective: To observe the effect of Hongjing Prescription on the phenotype of rat corpus cavernosum smooth muscle cells under hypoxic condition. Methods: One hundred and twenty SD rats were randomly divided into normoxia group, hypoxia model group, prostatic E1 group, Hongjing Ⅰ low dose group, Hongjing Ⅰ middle dose group, Hongjing Ⅰ high dose group , 20 in each group. The erectile status of rats in each group was measured. The relative expression of α-actin and OPN was determined by RT-PCR. Results: After six weeks of modeling, the number of erection in model group was significantly lower than that in normoxia group (P <0.01). The hypoxia experimental animal model was established successfully. Compared with normoxia group, the content of α-actin in hypoxia model group was significantly decreased (P <0.01) and OPN content was significantly increased (P <0.01). Compared with hypoxia model group, the content of α-actin in high school and low dose Hongjing Ⅰ group increased (P <0.05), and the contents of high and middle dose groups increased significantly (P <0.01); the content of OPN decreased (P <0.05) High school dose group decreased significantly (P <0.01). Conclusion: Hongjing Ⅰ Prescription can inhibit the phenotype transformation of sponge smooth muscle cells.