论文部分内容阅读
目的了解2012年云南省和缅甸籍入境健康儿童肠道病毒(Enterovirus,EV)带毒情况及病毒型别。方法采集6个边境设区的市(州、地区,下同)10个县和7个非边境市10个县<15岁健康儿童粪便标本600份(单份粪便标本,其中缅甸籍入境儿童100份),进行病毒分离和基因测序定型。结果600份粪便标本共检测到EV53株,带毒率为8.83%。其中脊髓灰质炎病毒5株,阳性率0.83%,均为疫苗株,未发现脊灰野病毒。检测到非脊灰肠道病毒(Non-polio EV,NPEV)48株,阳性率8.00%,其中人类肠道病毒(Human EV,HEV)A组5株(3个血清型,占10.42%),HEV B组(Group B)(HEV-B)42株(14个血清型,占87.50%),HEVC组6株(4个血清型,占11.32%),未分离到HEV D组。结论2012年600份健康儿童粪便标本中,从中国籍儿童分离到EV48株,分离率8.00%,缅甸籍儿童中分离到EV5株,分离率0.83%,中国籍儿童EV携带率较高,并以HEV-B组为主。
Objective To understand the infectious status and virus types of Enterovirus (EV) in entry-healthy children in Yunnan and Myanmar in 2012. Methods Six hundred faecal samples of healthy children aged <15 from 10 counties and 10 non-border cities were collected from 6 border cities (single stool samples, of which 100 were Myanmar nationals) Copies), virus isolation and gene sequencing stereotypes. Results A total of 600 stool samples were detected EV53 strains, with a virus rate of 8.83%. Among them, 5 were poliovirus, the positive rate was 0.83%. All of them were vaccine strains, and no poliovirus was found. 48 strains of non-polio EV (NPEV) were detected with a positive rate of 8.00%. Among them, 5 strains (3 serotypes, 10.42%) in group A of human EV (HEV) There were 42 strains of HEV-B (14 serotypes, 87.50%) and 6 (4 serotypes, 11.32%) of HEV group B, which were not isolated from HEV-D group. Conclusion Among 600 healthy children stool samples from 2012, EV48 isolates were isolated from Chinese children, the isolation rate was 8.00%. EV5 isolates were isolated from Burma children, the isolation rate was 0.83%. The prevalence of EV in Chinese children was higher than that in HEV -B group-based.