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miR398是受逆境胁迫负调控的miRNA,其靶基因CSD编码超氧化物歧化酶(SOD),使植物抵御活性氧(ROS)的毒害。为进一步了解低温胁迫下miR398的调控机制,从东农冬麦1号中克隆小麦miR398前体,构建过表达载体并转化拟南芥,用Real-time PCR检测T0代植株中小麦miR398及其靶基因CSD1在低温胁迫下的表达量。结果表明,随着低温胁迫时间的延长,miR398表达下调、CSD1基因表达上调,认为小麦miR398能响应低温胁迫、负调控CSD1基因表达,间接提高了拟南芥的抗寒性。
miR398 is a miRNA negatively regulated by stress and its target gene, CSD, encodes superoxide dismutase (SOD), which protects plants against reactive oxygen species (ROS). To further understand the regulation mechanism of miR398 under low temperature stress, we constructed the overexpression vector miR454 from Dongnong Dongmai 1 and transformed it into Arabidopsis thaliana. The miR398 and its target of T0 plants were detected by Real-time PCR Expression of gene CSD1 under low temperature stress. The results showed that the expression of miR398 was down-regulated and the expression of CSD1 was up-regulated with the prolonging of cold stress time. It was suggested that miR398 could negatively regulate the expression of CSD1 gene in response to low temperature stress, indirectly increasing the cold resistance of Arabidopsis thaliana.