EXPERIMENTAL STUDY OF THE EFFECT OF ANGIOGENESIS INHIBITOR TNP-470 ON THE GROWTH AND METASTASIS OF G

• 作 者: LIU Bing-ya LIN Yan-zhen YIN Hao-ran WANG Rui-nian ZHU Zheng-gang GU Qin-long
• 机 构: Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Second Medical University, Shangh
• 出 处: 中国癌症研究（英文版）
• 发表日期: 1999年1期
• 关键词: Gastric cancer TNP-470 Angiogenesis Liver metastasis Tumor growth Inhibition

Objective: To study the effect of angiogenesis inhibitor TNP-470 on the growth and metastasis of gastric cancer in vivo. Methods: Metastatic model simulating human gastric cancer was established by orthotopic implantation of histologically intact tumor tissue into gastric wall of nude mice. TNP-470 was administrated S.C. at doses of 0 mg/kg, 15 mg/kg, 30mg/kg, 60 mg/kg every other day for eight weeks. Ten weeks after implantation, the mice were sacrificed and the tumor size measured and the presence of metastasis recorded. The microvascular density was examined by immunohistochemical staining with anti-human factor Ⅷ antibody. Results: Compared to the untreated controls, growth of the orthotopically implanted tumor was significantly reduced in size in the mice treated with TNP-470 with an inhibition rate of 59.9%, 77.0% and 84.9% at the dosage of 15 mg/kg, 30 mg/kg and 60 mg/kg,respectively. Tumor metastasis to the liver and peritoneum was also significantly inhibited in a dosedependent manner. The microvascular density was also decreased significantly in the treated mice. Conclusion:Angiogenesis inhibitor TNP-470 has strong inhibitory effect both on tumor growth and metastasis of human gastric cancer in nude mice.