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应用标记Ｒ－Ｓ细胞、Ｂ淋巴细胞和Ｔ淋巴细胞的５种单克隆抗体ＬｅｕＭ_１、ＢｅｒＨ_２、ＬＣＡ、Ｌ_（２６）和ＵＣＨＬ_１对４３例何杰金病（ＨＤ）的石蜡标本进行了免疫组织化学染色，以Ｔ、Ｂ细胞淋巴瘤各１５例作对照研究。结果：非淋巴细胞为主型何杰金病（ｎｏｎＬＰＨＤ）的Ｒ－Ｓ细胞对ＬｅｕＭ_１的阳性率为６４．３％，对ＬＣＡ、Ｌ_（２６）和ＵＣＨＬ_１呈阴性反应；而非何杰金淋巴瘤（ＮＨＬ），ＬｅｕＭ_１仅偶在Ｔ细胞淋巴瘤中呈阳性（６．７％），ＬＣＡ、Ｌ_（２６）和ＵＣＨＬ_１标记ＮＨＬ、Ｂ－ＮＨＬ和Ｔ－ＮＨＬ阳性率分别是８３．３％、７３．３％和６６．７％。因此，在石蜡切片上联合应用ＬｅｕＭ１、ＬＣＡ、Ｌ_（２６）和ＵＣＨＬ_１对鉴别ＨＤ与ＮＨＬ有重要的参考价值。淋巴细胞为主型何杰金病（ＬＰＨＤ）的Ｒ－Ｓ细胞对ＬＣＡ和Ｌ_（２６）的阳性率为５３．３％和６０％，ｎｏｎＬＰＨＤ中Ｒ－Ｓ细胞对ＢｅｒＨ_２的阳性率为２１．４％。提示ＬＰＨＤ的Ｒ－Ｓ细胞来源于Ｂ淋巴细胞，ｎｏｎＬＰＨＤ的Ｒ－Ｓ细胞可能与活化的淋巴细胞有关。
Immunohistochemistry was performed on paraffin-embedded specimens of 43 Hodgkin’s disease (HD) samples using five monoclonal antibodies labeled with LeuM_1, BerH_2, LCA, L_ (26) and UCHL_1 labeled with R-S cells, B lymphocytes and T lymphocytes Staining, T, B-cell lymphoma in 15 cases as a control study. Results: The positive rate of LeuM_1 in non-lymphocytic non-Hodgkin’s disease (RIL) was 64.3%, negative for LCA, L_ (26) and UCHL_1, but not Hodgkin’s lymphoma The positive rates of NHL, LeuM_1 in T-cell lymphomas were 6.7%. The positive rates of NHL, B-NHL and T-NHL in LCA, L 26 and UCHL 1 were 83.3% 73.3% and 66.7%. Therefore, the combined application of LeuM1, LCA, L_ (26) and UCHL_1 on paraffin sections has important reference value for distinguishing between HD and NHL. The positive rates of LCA and L_ (26) in R-S cells of lymphoid-predominant Hodgkin’s disease (LPHD) were 53.3% and 60%, respectively. The positive rate of R-S cells to BerH_2 in nonLPHD was 21%. 4%. It is suggested that the R-S cells of LPHD are derived from B lymphocytes, and the R-S cells of nonLPHD may be related to activated lymphocytes.