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目的:观察β淀粉样蛋白(amyloid β-peptide,Aβ)神经毒性、血清诱导激酶(serum-inducible kinase,SNK)-树突棘相关Rap特异性GTP酶活化蛋白(spine-associated Rap guanosine triphosphatase activating protein,SPAR)途径、N-甲基-D-门冬氨酸受体(N-methyl-D-aspartate receptor,NMDAR)三者的关系,探讨滋补脾阴方药保护Aβ损伤原代培养大鼠海马神经元的作用机制。方法:将干粉Aβ1-40配制成溶液,在37℃恒温箱中孵育72h,获得聚集态纤维状Aβ1-40。原代培养大鼠海马神经元,以血清药理学方法制备滋补脾阴方药含药血清,建立Aβ1-40(5μmol/L)损伤神经元模型,滋补脾阴方药含药血清为干预组,采用逆转录聚合酶链式反应方法观察SNK、SPAR及NMDAR亚基NR1、NR2A、NR2B mRNA表达。结果:与对照组相比,神经元暴露于Aβ1-40(5μmol/L)2h后,SNKmRNA表达上调,SPAR、NR1、NR2A和NR2B mRNA表达下调,差异有统计学意义(P<0.01,P<0.05)。与Aβ1-40(5μmol/L)组比较,各浓度滋补脾阴方药含药血清组SNKmRNA表达下调,SPAR、NR1、NR2A和NR2B mRNA表达上调,以2%滋补脾阴方药含药血清效果最显著(P<0.05)。结论:Aβ引起神经元损伤的过程与SNK-SPAR途径和NMDAR相关;滋补脾阴方药对Aβ损伤神经元有保护作用,其保护机制可能与调节NMDAR表达,阻断SNK-SPAR途径有关。
Objective: To observe the amyloid β-peptide (Aβ) neurotoxicity, serum-inducible kinase (SNK), spine-associated Rap guanosine triphosphatase activating protein , SPAR) pathway, N-methyl-D-aspartate receptor (NMDAR), explore the effects of nourishing spleen and yin medicine on Aβ injury in primary cultured rat hippocampal nerve The mechanism of action of the yuan. METHODS: The dry powder Aβ1-40 was formulated into a solution and incubated in a 37°C incubator for 72 h to obtain aggregated fibrous Aβ1-40. Primary cultured rat hippocampal neurons were prepared by serum pharmacology with nourishing serum containing spleen and yin medicine. Aβ1-40 (5 μmol/L) damaged neuron model was established. Nourishing spleen and yin prescription drug-containing serum was used as the intervention group. The expression of NR1, NR2A and NR2B mRNA in SNK, SPAR and NMDAR subunits was observed by polymerase chain reaction. Results: Compared with the control group, SNK mRNA expression was up-regulated after exposure to Aβ1-40 (5 μmol/L) for 2 hours, and the mRNA expression of SPAR, NR1, NR2A, and NR2B was down-regulated. The difference was statistically significant (P<0.01, P< 0.05). Compared with Aβ1-40 (5μmol/L) group, the expression of SNK mRNA was down-regulated in each group of ZBZ serum containing serum, and the expression of SPAR, NR1, NR2A, and NR2B mRNA was up-regulated. The effect of 2% nourishing spleen-Yin prescription drug-containing serum was most significant. (P<0.05). Conclusion: The process of Aβ-induced neuronal injury is related to SNK-SPAR pathway and NMDAR. Nourishing spleen-yin decoction has a protective effect on Aβ-injured neurons, and its protective mechanism may be related to regulating NMDAR expression and blocking SNK-SPAR pathway.