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目的观察脑心通对氧化低密度脂蛋白(ox-LDL)作用下的人THP-1细胞活力及炎症反应的影响。方法将THP-1源性巨噬细胞分为7个组,即对照组(不加任何干预)、ox-LDL组(50mg/L ox-LDL干预细胞24h)、脑心通1组、脑心通2组、脑心通3组、脑心通4组(分别以0.125g/L、0.25g/L、0.5g/L、1.0g/L脑心通和50mg/L ox-LDL与细胞共孵育24h)、阿托伐他汀组(1μmol/L阿托伐他汀和50mg/L ox-LDL与细胞共孵育24h)。噻唑蓝(MTT)比色法检测THP-1细胞活力,酶联免疫吸附法(ELISA)检测细胞上清液中白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的含量。结果脑心通有轻度保护ox-LDL作用下巨噬细胞活力的作用,并抑制其引起的细胞炎症反应,上述作用均呈浓度依赖性,1g/L脑心通作用最为明显。结论脑心通呈浓度依赖性抑制ox-LDL引起的细胞损伤及炎症反应。
Objective To observe the effect of Naoxintong on the viability and inflammatory response of human THP-1 cells induced by oxidized low-density lipoprotein (ox-LDL). Methods THP-1 macrophages were divided into 7 groups: control group (without any intervention), ox-LDL group (50 mg/L ox-LDL intervention cells for 24 hours), Naoxintong group 1, brain heart Tong 2 group, Naoxintong 3 group, Naoxintong 4 group (0.125g/L, 0.25g/L, 0.5g/L, 1.0g/L Naoxintong and 50mg/L ox-LDL respectively Incubation 24h), atorvastatin group (1μmol/L atorvastatin and 50mg/L ox-LDL co-incubated with cells for 24h). The viability of THP-1 cells was detected by MTT colorimetric assay, and the levels of interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) in cell supernatants were determined by enzyme-linked immunosorbent assay (ELISA). . RESULTS: Naoxintong had mild protection of macrophage viability under the action of ox-LDL, and inhibited the inflammatory response induced by ox-LDL. These effects were concentration-dependent, and 1g/L brain-cardiac function was the most obvious. Conclusion Brain-heart-opening inhibits ox-LDL-induced cell injury and inflammation in a concentration-dependent manner.