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目的在荷瘤动物模型观察小鼠肝癌细胞(H22)来源的外泌体(exosomes)激发宿主抗肝癌免疫应答的效应。方法用自行分离纯化的小鼠肝癌细胞H22源外泌体免疫小鼠,然后给小鼠皮下注射H22肿瘤细胞,观察肿瘤的生长状况。用MTT法检测小鼠脾细胞增殖情况和细胞毒活性;用免疫组织化学法检测肿瘤组织中CD4+、CD8+T淋巴细胞浸润情况。结果该外泌体使肿瘤出现时间延迟,肿瘤生长缓慢,小鼠生存率提高。促进脾淋巴细胞增殖并增强其细胞毒活性,促进CD4+和CD8+T细胞活化。结论小鼠肝癌细胞(H22)源外泌体能诱导抗肿瘤应答,有效地诱导特异性的杀伤肿瘤细胞活性,有针对亲本细胞的免疫保护作用。
OBJECTIVE: To observe the effect of exosomes derived from mouse hepatoma cells (H22) on host tumor-inducing immune responses against hepatocellular carcinoma in a tumor-bearing animal model. Methods Mice were immunized with H22-derived exosomes of mouse hepatocarcinoma cells. H22 tumor cells were injected into mice subcutaneously to observe the growth of the tumor. The proliferation and cytotoxicity of mouse spleen cells were detected by MTT assay. The infiltration of CD4 + and CD8 + T lymphocytes in tumor tissues was detected by immunohistochemistry. Results The exosomes delayed tumor appearance, slow tumor growth, and improved survival in mice. Promote spleen lymphocyte proliferation and enhance its cytotoxic activity, and promote the activation of CD4 + and CD8 + T cells. Conclusions The exosomes derived from mouse hepatoma cells (H22) can induce antitumor responses and effectively induce specific cytotoxicity against tumor cells, and have immunoprotective effects against the parental cells.