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目的:观察趋化因子白介素-8(Interleukin-8,IL-8)基因单核苷酸多态性与急性冠脉综合征(acute coronarysyndrome,ACS)的相关性。方法:采用直接测序的方法对675例ACS的患者和636例对照组进行检测,分析IL-8基因+394T/G单核苷酸多态的基因型和等位基因频率的分布情况。结果:IL-8基因+394 T/G单核苷酸多态在ACS组和对照组间的分布频率皆符合Hardy-Weinberg平衡定律,IL-8基因+394 T/G单核苷酸多态三种基因型(GG型,GT型和TT型)在ACS组分布频率分别为16.9%,48.7%和34.4%,在对照组分别为18.1%,50.9%和31.0%。IL-8基因+394 T/G多态基因型和等位基因频率在正常对照组和ACS组之间无明显的相关(P>0.05)。Logistic回归校正性别、年龄、体质量指数、吸烟、高血压病、高脂血症、糖尿病等冠心病易患因素后,IL-8基因+394T/G多态与ACS的发病无相关关系。结论:在中国北方汉族人群中IL-8基因+394T/G多态与ACS发病无相关关系,IL-8基因+394 T/G多态不是ACS发病的独立危险因素。
Objective: To investigate the association between Interleukin-8 (IL-8) gene single nucleotide polymorphism and acute coronary syndrome (ACS). Methods: Six hundred and seventy-six patients with ACS and 636 controls were examined by direct sequencing. The distribution of genotype and allele frequency of IL-8 + 394T / G SNP was analyzed. Results: The frequency of +394 T / G SNP in IL-8 gene was in accordance with Hardy-Weinberg equilibrium law and IL-8 gene +394 T / G single nucleotide polymorphism The frequencies of the three genotypes (GG, GT and TT) were 16.9%, 48.7% and 34.4% in the ACS group and 18.1%, 50.9% and 31.0% in the control group, respectively. The genotype and allele frequency of IL-8 +394 T / G polymorphism were not significantly different between ACS group and control group (P> 0.05). Logistic regression showed no correlation between IL-8 gene + 394T / G polymorphism and the incidence of ACS after correction of the risk factors such as gender, age, body mass index, smoking, hypertension, hyperlipidemia and diabetes. Conclusion: There is no correlation between IL-8 + 394T / G polymorphism and ACS in Chinese Han population in northern China. IL-8 +394 T / G polymorphism is not an independent risk factor for ACS.