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目的探讨影响脑白质疏松(leukoaraiosis,LA)形成的相关因素,并分析亚甲基四氢叶酸还原酶(MTHFR)基因多态性对LA发病的影响。方法收集2015年1月~2016年3月在我院接受治疗的134例LA患者作为LA组和73例健康体检的老年人作为对照组,收集受试者的一般临床资料并进行相关生化检测。对照组70例、LA组131例患者进行MTHFR的2个基因C667T及A1298CPCR扩增和基因多态性分析。结果 logistic回归分析显示,年龄(95%CI:1.877~5.134,P=0.000)、高血压(95%CI:1.223~4.631,P=0.011)、同型半胱氨酸(95%CI:1.647~6.090,P=0.001)为LA的独立危险因素。2组患者C677T不同基因分型和碱基比例比较,差异无统计学意义(P>0.05)。A1298C不同基因分型和碱基比例比较,差异有统计学意义(P<0.05)。结论 LA与MTHFR C667T基因多态性无相关,MTHFR A1298C基因突变可能与LA的发病相关。
Objective To investigate the related factors that affect the formation of leukoaraiosis (LA) and analyze the influence of MTHFR polymorphism on the pathogenesis of LA. Methods A total of 134 LA patients treated in our hospital from January 2015 to March 2016 were selected as the LA group and 73 healthy controls as the control group. The general clinical data and biochemical tests were collected. In the control group, 70 patients in the LA group and 131 patients in the LA group were subjected to C667T and A1298CPCR amplification of 2 genes of MTHFR and genetic polymorphism analysis. Results Logistic regression analysis showed that age (95% CI 1.877-5.134, P 0.000), hypertension (95% CI 1.223-4.631, P 0.011), homocysteine 95% CI 1.647-6.090 , P = 0.001) were independent risk factors for LA. There was no significant difference in genotypes and base ratios of C677T between the two groups (P> 0.05). A1298C different genotyping and base ratio, the difference was statistically significant (P <0.05). Conclusion There is no correlation between LA and MTHFR C667T gene polymorphism. MTHFR A1298C gene mutation may be related to the pathogenesis of LA.