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目的探讨散发腹泻患者粪便中肠致病性大肠埃希菌的分离鉴定。方法依据GB/T4789.6-2003和WS271-2007对1例腹泻患者粪便标本进行培养分离,经革兰染色镜检、生化试验、血清学试验作出鉴定。结果从腹泻患者粪便中分离鉴定出肠致病性大肠埃希菌EPEC O142:K86(B)。结论导致该患者腹泻的病原菌由肠致病性大肠埃希菌引起。
Objective To investigate the isolation and identification of enteropathogenic Escherichia coli in feces of diarrhea patients. Methods According to GB / T4789.6-2003 and WS271-2007, one stool specimen of diarrhea patients were cultured and separated, and identified by Gram stain, biochemical test and serological test. RESULTS: Enteropathogenic Escherichia coli EPEC O142: K86 (B) was isolated and identified from diarrhea patients. Conclusion The pathogen causing diarrhea in this patient is caused by enteropathogenic Escherichia coli.