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目的探讨ｒａｓ癌基因在大肠腺瘤、大肠癌组织中的表达及意义。方法应用免疫组织化学ＬＳＡＢ法，检测５４例大肠癌、１７例大肠腺瘤和３１例正常粘膜中的ｒａｓｐ２１的分布。结果癌组织及腺瘤组织中均有ｒａｓｐ２１过量表达，阳性率分别为５７．４％和２９．４％，均高于正常粘膜（Ｐ＜０．０５），在大肠癌淋巴结转移组ｐ２１阳性表达高于非淋巴结转移组（Ｐ＜０．０５）。结论ｒａｓｐ２１的过量表达与大肠癌的发生及淋巴结转移有关。ｒａｓｐ２１在大肠腺瘤不典型增生上皮中的高表达，可作为早期癌变的参考诊断指标，大肠癌ｐ２１表达阳性率与癌分化程度、浸润深度无明显关系
Objective To investigate the expression and significance of ras oncogene in colorectal adenoma and colorectal cancer. Methods The expression of rasp21 in 54 cases of colorectal cancer, 17 cases of large intestine adenoma and 31 cases of normal mucosa was detected by immunohistochemical LSAB method. RESULTS: Overexpression of rasp21 was detected in both cancer tissues and adenomas. The positive rates were 57.4% and 29.4%, respectively, which were higher than those in normal mucosa (P<0.05). The expression of p21 was positive in the lymph node metastasis group. Higher than non-lymph node metastasis (P<0.05). Conclusion The overexpression of rasp21 is associated with the occurrence of colorectal cancer and lymph node metastasis. The high expression of rasp21 in atypical hyperplasia epithelium of colorectal adenoma can be used as a reference diagnostic indicator for early canceration. The positive rate of p21 expression in colorectal cancer is not significantly related to the degree of differentiation and depth of invasion.