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为观察窒息新生儿血清血管内皮生长因子(VEGF)水平的动态变化并探讨其临床意义,我们应用双抗体夹心酶联免疫吸附法(ELISA)对正常新生儿(对照组30例)、窒息新生儿(窒息组47例)出生后即刻、第1天和第3天3个时间点血清VEGF水平进行检测。结果显示窒息组出生时血清VEGF水平显著上升,与对照组相比有极显著的差异(P<0.01),其水平上升的程度与新生儿窒息的严重程度有关,与血pH值及1分钟Apgar评分成负相关(P<0.05)。出生后第1天,轻度窒息组血清VEGF水平已明显下降。与对照组无显著性差异(P>0.05);重度窒息组血清VEGF水平虽然与对照组相比仍有显著性差异,但也出现下降趋势。到出生后第3天,窒息组与对照组相比已无显著性差异(P>0.05)。上述结果表明,动态检测窒息新生儿血清VEGF水平可望成为反映新生儿窒息程度以及转归的一个敏感和特异的指标,同时提示它可能在新生儿窒息的病理生理过程中发挥作用。
To observe the dynamic changes of serum vascular endothelial growth factor (VEGF) levels in asphyxia neonates and its clinical significance, we used double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the changes of normal neonatal (control group, 30 cases) (Asphyxia group 47 cases) immediately after birth, the first day and the third day of three time points to detect serum VEGF levels. The results showed that serum VEGF level in asphyxia group was significantly higher than that in control group (P <0.01). The level of serum VEGF level was related to the severity of neonatal asphyxia, The scores were negatively correlated (P <0.05). On the first day after birth, the level of serum VEGF in mild asphyxia group was significantly decreased. (P> 0.05). The level of serum VEGF in severe asphyxia group was still significantly lower than that of the control group (P> 0.05). On the third day after birth, no significant difference was found between asphyxia group and control group (P> 0.05). The above results indicate that the dynamic detection of serum VEGF levels in neonates with asphyxia is expected to become a sensitive and specific indicator of neonatal asphyxia and prognosis, suggesting that it may play a role in the pathophysiology of neonatal asphyxia.