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目的探讨人胰腺癌血管生成方式和时间，分析其临床意义，为寻求抗人胰腺癌血管生成药物做准备。方法用SCID鼠制做皮肤血管室模型，血管室内接种人胰腺癌细胞株，活体显微镜下连续观察2周，录制图像，使用图像分析软件比较血管面积、密度及直径的变化。结果1周内新生血管基本完成，2周内形成肉眼可见的微小肿瘤；血管密度和面积的变化显著（P＜0.001，P＜001）；血管直径的变化差异无显著性（P＞0.05）。结论人胰腺癌细胞可诱导周围正常血管内皮细胞增生形成肿瘤血管；胰腺癌术后的辅助治疗应开始于术后2周以内；血管密度可作为估计胰腺癌恶性程度高低的生物学指标。
Objective To investigate the angiogenesis pattern and time of human pancreatic cancer, analyze its clinical significance, and prepare for antiangiogenic drugs against human pancreatic cancer. Methods The skin vascular chamber model was established with SCID mice. The human pancreatic cancer cell lines were inoculated intratumorally and observed continuously for 2 weeks under a living microscope. The images were recorded and the changes in the area, density, and diameter of blood vessels were compared using image analysis software. Results The neovascularization was basically completed within 1 week and macroscopic tumors were formed within 2 weeks. The changes of vascular density and area were significant (P<0.001, P<001). There was no significant difference in the change of vessel diameter (P>0.05). Conclusion Human pancreatic cancer cells can induce the formation of tumor blood vessels by proliferation of normal vascular endothelial cells. The adjuvant treatment of pancreatic cancer should begin within 2 weeks after surgery. The vascular density can be used as a biological indicator to estimate the degree of malignancy of pancreatic cancer.