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目的探讨白细胞介素８（ＩＬ８）及其Ａ、Ｂ受体（ＩＬ８ＲＡ、ＩＬ８ＲＢ）的表达与急性白血病（ＡＬ）的分型、疗效及并发症的关系。方法用酶联免疫夹心法检测７７例初诊ＡＬ患者外周血ＩＬ８含量，用间接免疫荧光及流式细胞术检测骨髓单个核细胞（ＭＮＣｓ）表达ＩＬ８Ｒ，并检测了１５例ＡＬ完全缓解（ＣＲ）期脑脊液（ＣＳＦ）中ＩＬ８水平。结果ＡＬ患者外周血ＩＬ８水平高于正常人，其中急性髓系白血病（ＡＭＬ）高于急性淋巴细胞白血病（ＡＬＬ），Ｍ４、Ｍ５高于Ｍ１～Ｍ３，ＢＡＬＬ高于ＴＡＬＬ（Ｐ＜０．０５）。ＡＬＬ中ＩＬ８＞１００ｎｇ／Ｌ组比≤１００ｎｇ／Ｌ组疗效差（Ｐ＜０．０５）；约３６．３６％的ＡＬ患者ＩＬ８Ｒ（＋），ＩＬ８Ｒ（＋）者的外周血白细胞计数及幼稚细胞比例显著高于ＩＬ８Ｒ（－）者（Ｐ＜０．０５）；ＣＲ期患者ＣＳＦ中ＩＬ８水平与治疗前比较，差异无显著性（Ｐ＞０．０５），合并中枢神经系统白血病（ＣＮＳＬ）时明显升高。结论外周血和ＣＳＦ中ＩＬ８水平的检测及检测骨髓ＭＮＣｓ膜ＩＬ８Ｒ的表达有助于ＡＬ各亚型的鉴别及预后判断；ＣＮＳＬ发生时ＣＳＦ中ＩＬ８增高。
Objective To investigate the relationship between the expression of interleukin-8 (IL-8) and its A and B receptors (IL-8RA, IL-8RB) and the type, efficacy and complications of acute leukemia (AL). Methods IL-8 levels in peripheral blood of 77 patients with newly diagnosed AL were detected by enzyme-linked immunosorbent assay. IL-8R in bone marrow mononuclear cells (MNCs) was detected by indirect immunofluorescence and flow cytometry, and 15 AL complete remissions were detected. IL-8 levels in (CR) cerebrospinal fluid (CSF). Results IL-8 levels in peripheral blood of AL patients were higher than normal subjects. Among them, acute myeloid leukemia (AML) was higher than acute lymphoblastic leukemia (ALL), M4, M5 were higher than M1 ~ M3, B-ALL was higher than T-ALL ( P<0.05). The efficacy of IL-8>100ng/L group≤100ng/L group in ALL was poor (P<0.05); about 36.36% of AL patients had IL-8R(+) and IL-8R(+) peripherals. The white blood cell count and proportion of naive cells were significantly higher than those of IL-8R(-) (P<0.05); the levels of IL-8 in CSF patients at CR stage had no significant difference compared with that before treatment (P>0.05). , when combined with central nervous system leukemia (CNSL) increased significantly. Conclusions The detection of IL-8 levels in peripheral blood and CSF and the detection of IL-8R expression in bone marrow MNCs contribute to the identification and prognosis of AL subtypes; the increase of IL-8 in CSF during CNSL occurs.