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目的建立人血浆中头孢噻肟的含量测定方法。方法采用高效液相色谱法测定,用Luna C18柱(250×4.6mm,5μm),以三乙胺水溶液(1g/L,磷酸调pH=5.0)甲醇乙腈(80 7 13)为流动相,检测波长254nm。结果头孢噻肟钠的线性范围为1.000mg/L-125.0mg/L,相关系数r=0.9999,最低检测限为1.000mg/L,低、中、高剂量平均回收率分别为91.6%、86.6%、93.5%,RSD分别为2.4%、0.6%、7.9%(n=5)。结论所用方法测定样品灵敏度高,重复性好,能有效地测量人血浆中头孢噻肟的含量。
Objective To establish a method for the determination of cefotaxime in human plasma. Methods High performance liquid chromatography (HPLC) was used. The mobile phase consisted of acetonitrile (80 7 13) with Luna C18 column (250 × 4.6 mm, 5 μm) and triethylamine in 1 g / L phosphoric acid Wavelength 254nm. Results The linear range of cefotaxime sodium was 1.000mg / L-125.0mg / L, the correlation coefficient was 0.9999, the lowest detection limit was 1.000mg / L, the average recoveries were 91.6%, 86.6% , 93.5% with RSD of 2.4%, 0.6% and 7.9% respectively (n = 5). Conclusion The method used to determine the sample sensitivity, repeatability, and can effectively measure the content of cefotaxime in human plasma.