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本文对山东埕口盐场、海南岛莺歌海盐场及青海柯柯盐湖天然采集的卤虫卵进行了孵化特性研究。埕口、莺歌海及柯柯的虫卵壳厚分别为9.7,11.4及13.2μm。在放入海水中进行孵化前上述三品系的卤虫卵分别在自来水中浸泡2,3及4小时能获得最佳孵化率。它们的最适孵化温度分别为25.6~28.5℃,27.0~30.0℃及27.0~33.0℃。最适孵化盐度分别为30,20及35%。孵化的同步性以“莺歌海”为最好,为6.4小时,“埕口”次之,为6.5小时,“柯柯”为7.4小时。 实验室内培养产卵后24小时内收集的虫卵用下列方法处理能终止虫卵的滞育状态:(1)埕口卤虫卵:浸泡于粗盐饱和溶液中2~3个月,孵化率可达91~94%。(2)莺歌海卤虫卵:浸泡于粗盐饱和溶液中2~5个月,孵化率可达80~91%。(3)柯柯卤虫卵:置于5℃2~3个月孵化率可达96~98%。用3%H_2O_2及3%甲醛溶液处理对终止滞育状态没有作用。天然采集的三个品系的卤虫卵用3%H_2O_2或3%甲醛溶液或用去壳溶液处理均可以提高孵化率。
In this paper, hatching characteristics of brine shrimp collected from Shandongkoukou salt field, Hainan Island Yinggehai salt field and Qinghai Keke salt lake were studied. Yingkou, Yinggehai and Keke eggshell thickness were 9.7,11.4 and 13.2μm. Artemia eggs of these three lines were incubated in tap water for 2, 3, and 4 hours before they were hatched in seawater to obtain the best hatchability. Their optimum incubation temperature were 25.6 ~ 28.5 ℃, 27.0 ~ 30.0 ℃ and 27.0 ~ 33.0 ℃. The optimal hatching salinity was 30, 20 and 35% respectively. Incubation synchronization is “Yinggehai” is the best, 6.4 hours, “mouth” followed by 6.5 hours, “Ke Ke” is 7.4 hours. Intra-laboratory cultivation of eggs collected within 24 hours after spawning The diapause of eggs can be terminated by the following methods: (1) brine shrimp eggs: soaked in salt-saturated solution for 2 to 3 months and hatched Rate up to 91 ~ 94%. (2) Yinggehai Artemia eggs: immersed in salt-saturated solution for 2 to 5 months, the hatching rate of up to 80 to 91%. (3) Ke Ke Artemia eggs: placed at 5 ℃ 2 to 3 months hatching rate of 96 to 98%. Treatment with 3% H 2 O 2 and 3% formaldehyde solution had no effect on termination of diapause. The hatching rate of Artemia salina eggs of three strains collected naturally with 3% H 2 O 2 or 3% formaldehyde solution or with shelled solution can be increased.