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选择乙型肝炎 (乙肝 )病毒核心抗原 (HBcAg)、e抗原 (HBeAg)及单纯疱疹病毒 gD抗原 (HSV - 1-gD)基因为目的基因 ,进行DNA疫苗联合免疫的研究。通过对不同基因片段的表达研究 ,选择了能在哺乳动物细胞中高效表达乙肝病毒核心抗原、e抗原和单纯疱疹病毒 gD抗原的质粒DNA免疫Balb/c小鼠。结果显示 :表达乙肝病毒核心抗原和单纯疱疹病毒gD抗原的DNA疫苗单独免疫 ,能有效刺激机体产生体液免疫和细胞免疫 ,二者的联合免疫明显加强了这种免疫效果 ,并可以使乙肝核心抗体提前出现。但表达乙肝病毒e抗原和单纯疱疹病毒 gD抗原的DNA疫苗联合免疫则加强作用不明显。这些结果表明 :不同DNA疫苗的适当组合有可能在获得多价疫苗的同时 ,提高DNA疫苗的免疫效果。
The HBcAg, e antigen (HBeAg) and herpes simplexoviridae gD antigen (HSV - 1 - gD) were selected as the target genes to study the DNA vaccine combined immunization. Balb / c mice were immunized with plasmid DNA that could express hepatitis B virus core antigen, e antigen and herpes simplex virus gD antigen efficiently in mammalian cells by studying the expression of different gene fragments. The results showed that the DNA vaccine expressing hepatitis B virus core antigen and herpes simplex virus gD antigen alone could effectively stimulate the humoral and cellular immunity of the body, and the combination of the two could obviously enhance the immune effect and make the hepatitis B core antibody Appear in advance However, the combined immunization with DNA vaccine expressing hepatitis B virus e antigen and herpes simplex virus gD antigen showed no obvious effect. These results indicate that the appropriate combination of different DNA vaccines may increase the immunization efficacy of DNA vaccines while obtaining multivalent vaccines.