论文部分内容阅读
对同源四倍体高粱品系和杂交种的细胞学观察发现,其减数分裂的各个时期均有特异性。具体表现在,前期Ⅰ有3.94%的PMC中存在多个核仁,其中以双核仁形式出现的频率最高;终变期—中期Ⅰ具有不同的染色体构型,可观察到同源染色体的四种组成形式;后期Ⅰ出现大量落后染色体,其中以单价体提前分离造成的染色单体落后较为常见,单价染色体落后较少;前期Ⅱ和四分体时期可观察到核外染色体;中期Ⅱ发现有部分染色体不排列于赤道板上;后期Ⅱ仍有新的落后染色体产生。对四价体频率和后期Ⅰ带有落后染色体细胞出现频率进行的统计分析发现,品系间和不同的杂交种间存在显著的差异,说明染色体的配对能力和正常分配能力均受基因型影响。研究还发现,这两个重要的细胞学现象具有明显的负向超亲优势,杂交种的四价体频率明显降低,落后染色体明显减少。
Cytological observation of tetraploid tetraploid sorghum lines and hybrids showed that all stages of meiosis were specific. Specifically, there were 3.92% of PMCs in pre-Ⅰ, of which there were multiple nucleolus, of which the highest frequency appeared in the form of double-nucleolus. The metaphase Ⅰ had different chromosomal configurations in the terminal metaphase Ⅰ, and homologous chromosomes There were a large number of backward chromosomes in the late stage Ⅰ. Among them, the chromatid lagging caused by the premature separation of monovalent bodies was more common, and the monovalent chromosomes lagged behind. The extranodal chromosomes were observed in the early stage Ⅱ and the fourth stage. Some chromosomes are not arranged on the equatorial plate; late Ⅱ there are still new backward chromosomes. The statistical analysis of the frequency of tetravalent and the frequency of backward cells with lagging chromosomes in late stage Ⅰ showed that there were significant differences among the strains and among the different hybrids, indicating that both chromosome pairing ability and normal distribution ability are affected by genotypes. The study also found that these two important cytological phenomena have obvious negative super-affinity, hybrids significantly reduced the frequency of tetrads, lagging chromosomes significantly reduced.