论文部分内容阅读
为探讨皮肤鳞状细胞癌 (鳞癌 )和基底细胞癌 (基癌 )癌细胞上是否低水平表达 CD54抗原，应用低温聚合、包埋后染色的胶体金免疫电镜技术，对 3例鳞癌和 3例基癌 CD54免疫组化染色阴性的癌细胞进行了研究。在 3例鳞癌和 3例基癌中，各 2例癌细胞表面及突起处观察到 CD54胶体金颗粒的存在；其中 1例鳞癌张力微丝-桥粒复合体处还可见 CD54胶体金颗粒分布。研究发现，虽然鳞癌和基癌癌细胞 CD54免疫组化染色阴性，但应用胶体金免疫电镜可以观察到其癌细胞低水平表达 CD54抗原。该结果可解释皮肤鳞癌和基癌临床上可发生转移，但发生率较低的机理，值得进一步研究。
To investigate whether low-level expression of CD54 antigen on squamous cell carcinoma (squamous cell carcinoma) and basal cell carcinoma (basic cell carcinoma) cancer cells, low-temperature polymerization and post-embedding colloidal gold immunoelectron microscopy was performed on 3 cases of squamous cell carcinoma and Three cases of cancer cells that were negative for CD54 immunohistochemical staining were studied. In 3 cases of squamous cell carcinoma and 3 cases of basal carcinoma, the presence of CD54 colloidal gold particles was observed on the surface and projections of 2 cases of cancer cells; CD54 colloidal gold particles were also seen in 1 case of squamous cell carcinoma tension microfilaments-mitochondrial complex. distributed. The study found that although squamous cell carcinoma and base cancer cell CD54 were immunohistochemically negative, the use of colloidal gold immunoelectron microscopy allowed the observation of low-level expression of CD54 antigen in cancer cells. This result can explain the skin metastasis in squamous cell carcinoma and basal carcinoma, but the mechanism of low incidence is worthy of further study.