论文部分内容阅读
目的：通过胸腺内注射表达外源性主要组织相容性抗原复合物（MHC）抗原质粒PXN（N2－B19－H－2Kb），诱导异基因小鼠心肌移植耐受。方法：给BALB／C小鼠胸腺内注射质粒PXN（N2－B19－H－2Kb），将外源性的编码C57BL／6小鼠MHCI类抗原的H－2KbcDNA转移到BALBC／C小鼠胸腺，2周后行C57BL／6小鼠心肌移植。用聚合酶链反应（PCR），反转录聚合酶链反应（RT－PCR），单克隆抗体免疫萤光染色流式细胞仪检测BALB／C小鼠胸腺细胞DNA，mRNA和MHC蛋白质表达。结果：BALB／C小鼠胸腺细胞表面有外源性MHC分子表达，转染效率为5．1％，胸腺内注射质粒PXN（N2－mg－H－2Kb）能明显延长移植小鼠心肌的存活时间，平均17d，对照为8d（P＜0．01）。结论：同种MHC基因转移至受体鼠胸腺可以诱导特异性的免疫耐受。
OBJECTIVE: To induce myocardial allograft tolerance in allogeneic mice by intra-thymus injection of exogenous MHC antigen plasmid PXN (N2-B19-H-2Kb). METHODS: Plasmid PXN (N2-B19-H-2Kb) was injected into the thymus of BALB / C mice and the exogenous H-2Kb cDNA encoding the MHC class I antigens of C57BL / 6 mice was transferred to the thymus of BALBC / C57BL / 6 mice were transplanted after 2 weeks. The DNA, mRNA and MHC protein expression in thymocytes of BALB / C mice were detected by polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (RT-PCR) and monoclonal antibody immunofluorescence staining. Results: The expression of exogenous MHC on the surface of thymocytes in BALB / C mice was 5.1%, and the transfection efficiency was 5.1%. The intrathymic injection of PXN (N2-mg-H-2Kb) could prolong the myocardial viability Time, an average of 17d, the control was 8d (P <0.01). CONCLUSION: Allogeneic MHC gene transfer to recipient rat thymus can induce specific immune tolerance.