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目的用生物可降解聚乳酸羟基乙酸共聚物(PLGA)制备载药微球包埋血管内皮生长因子(VEGF),并探索不同配比对释放行为的影响。方法采用不同分子量的PLGA制备不同粒径的载药微球,并经载药微球的合理配比改善其体外释放行为,达到优化工艺、降低成本的目的。结果载药微球粒径约为20μm、分子量10 kU:24 kU的配比为1:2组,粒径为20μm、分子量为24 kU和分子量为10 kU、粒径为6μm的载药微球配比为2:1组的体外释放突释较低,且在14 d内呈线性的零级释放趋势,体外释放行为得到改善。结论 VEGF长效缓释PLGA微球经优化配比后的持续释放能力较传统VEGF微球明显提高。
OBJECTIVE: To prepare drug-loaded microspheres for embedding vascular endothelial growth factor (VEGF) with biodegradable polylactide glycolic acid copolymer (PLGA) and explore the effect of different ratios on the release behavior. Methods PLGA with different molecular weight was used to prepare drug-loaded microspheres with different particle sizes. The drug-loaded microspheres were modified with drug-loaded microspheres to improve their in vitro release behavior and achieve the goal of optimizing technology and reducing cost. Results The diameter of drug-loaded microspheres was about 20μm. The drug-loaded microspheres with a molecular weight of 10 kU: 24 kU were 1: 2, the particle size was 20 μm, the molecular weight was 24 kU, the molecular weight was 10 kU and the particle size was 6 μm The ratio of 2: 1 group in vitro release burst release is low, and in the 14 d linear zero-order release trends, in vitro release behavior was improved. Conclusion The sustained-release capacity of PLGA microspheres with long-acting sustained release of VEGF is obviously higher than that of traditional VEGF microspheres.