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大量的在体和离体实验证明，骨骼肌内存在着促进脊髓神经元存活和再生的物质。本研究采用高效液相分析方法对胎鼠、成鼠和人胚的骨骼肌提取液进行了分析。结果表明，三者在相同条件下尽管出现蛋白峰的数量不同，峰值也有所差异，但在相同的时间点出现两处４ 峰（Ⅰ、Ⅱ１、Ⅱ２、Ⅱ３）的基本波型。峰Ⅱ１ 的分子量为３５０００Ｄ，峰Ⅱ２ 的分子量为２２０００Ｄ，峰Ⅱ３ 的分子量为１８０００Ｄ。胎鼠和人胚的三峰呈中、低、高的形态分布，而成鼠的三峰呈低、中、高的形态分布。肌源神经营养因子（１０～３０ ｋＤ）位于峰Ⅱ。此结果使人们对肌源神经营养因子的存在产生了疑问，肌源神经营养因子究竟是一个新的营养因子（分子量究竟是多少？），还是已知的神经营养因子（ＣＮＴＦ，ＢＤＮＦ，ＮＴ３ 等）在骨骼肌组织中的分布？ 对此有必要进行分子生物学的研究来确定。
A large number of in vivo and in vitro experiments have shown that skeletal muscle exists to promote the survival and regeneration of spinal cord neurons. In this study, we used high performance liquid chromatography to analyze skeletal muscle extracts of fetal, adult and human embryos. The results showed that although the number of protein peaks and the peaks were different under the same conditions, there were two basic patterns of four peaks (Ⅰ, Ⅱ1, Ⅱ2 and Ⅱ3) at the same time point. The peak II1 has a molecular weight of 35000D, the peak II2 has a molecular weight of 22000D and the peak II3 has a molecular weight of 18000D. The three peaks of fetal and human embryos showed medium, low and high morphological distribution, while the three peaks of adult mice showed low, medium and high morphological distribution. Myogenic neurotrophic factor (10 ~ 30 kD) located in the peak Ⅱ. This result raised questions about the existence of myotrophic neurotrophic factors, whether it is a new trophic factor (what is the molecular weight?) Or known neurotrophic factors (CNTF, BDNF, NT 3, etc.) in skeletal muscle tissue distribution? It is necessary to carry out molecular biology research to determine.