论文部分内容阅读
运用人类高分辨染色体显微切割、ＰＣＲ和微克隆技术构建的８ｑ２４．１带特异性ｐＵＣ１９文库，筛选出一个含ＣＡ重复的新的多态微卫星ＤＮＡ［（ＣＡ）ｎ］。经ＰＣＲ检测人鼠杂种细胞板，证实其来源于人８号染色体；在正常人群中检出１１个等位片段，杂合度为０．８４；在１１个家系中进行连锁分析。结果显示，此多态标记与三个已知的位于８ｑ２３－２４．１区域的微卫星ＤＮＡ（Ｄ８Ｓ８５，Ｄ８Ｓ１９９，Ｄ８Ｓ２８１）紧密连锁，其在８号染色体遗传图谱上的相对位置为：着丝粒－Ｄ８Ｓ８５－新（ＣＡ）ｎ－Ｄ８Ｓ１９９－长臂末端。
A novel polymorphic microsatellite DNA [(CA) n] containing CA repeats was screened using 8q24.1 band-specific pUC19 library constructed by human high-resolution chromosome microdissection, PCR and microcoloning. The hybridization of human and mouse cell lines was confirmed by PCR, which was confirmed by the human chromosome 8. In the normal population, 11 alleles were detected with a heterozygosity of 0.84. A linkage analysis was performed in 11 pedigrees. The results showed that the polymorphic marker was closely linked to three known microsatellite DNAs (D8S85, D8S199, D8S281) located in the region of 8q23-24.1. The relative positions on the chromosome 8 genetic map were: centromere -D8S85-NEW (CA) n-D8S199-Long Arm Tip.