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在第三次三尖杉协作会上,根据化学、药理及临床的初步研究,提出了重点研究三尖杉生物碱中三尖杉酯碱对白血病的疗效。从三尖杉属植物中提取三尖杉酯碱,过去报导均采用不同pH 梯度法提取后用氧化铝柱层析或用逆流分配法分离。由于三尖杉中含多种生物碱,而酯碱含量较低,因而用氧化铝柱层析时洗脱时间长,消耗溶媒多,分离效果不理想,需要通过2次层析,这给较大量提取分离酯碱工作带来了困难。本文则采用氧化铝干柱层析法,从三尖杉中分离三尖杉酯碱,干柱层析是一种改进的层析技术,是在薄层层析及柱层析的基础上发展起来的。在国内应用干柱法分离植物有效成分尚未见报导。干柱法主要特点是在干柱上能很迅速地进行制备性分离,一般展开只
At the third conference on harringtonine, based on preliminary studies of chemistry, pharmacology, and clinical research, a focus was placed on the efficacy of harringtonine in harringtonine alkaloids on leukemia. The extraction of harringtonine from cephalocephalus has been reported in the past using different pH gradient extractions followed by alumina column chromatography or countercurrent distribution. Because harringtonia contains many alkaloids, and the content of ester base is low, the elution time is long when using alumina column chromatography, which consumes more solvent and the separation effect is not ideal. It needs to pass 2 times of chromatography, which gives The large amount of extraction and separation of ester base work has brought difficulties. In this paper, alumina dry column chromatography was used to separate the harringtonine from harringtonia. Dry column chromatography is an improved chromatographic technique developed on the basis of thin layer chromatography and column chromatography. Up. The use of dry column method to separate effective plant components in China has not yet been reported. The main feature of the dry column method is that the preparation column can be prepared very quickly on the dry column.