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乙型肝炎表面抗体(抗-HBs)是反映机体对乙型肝炎表面抗原(HBsAg)免疫能力的指标。我们以基因工程技术获得表达人源性抗—HBs Fab片段的工程化大肠杆菌,发酵表达该抗体的可溶性Fab片段,用固相放射免疫分析(SPRIA)定量测定Fab纯化晶的抗-HBs活性,结果如下。 材料和方法 一、人源性抗-HBs Fab抗体的制备:工程化人源性抗-HBs Fab片段的制备参见文献报道,并用抗-HBs阳性病员血清同法纯化作为对照。
Hepatitis B surface antibody (anti-HBs) is an indicator of the body’s immunity to hepatitis B surface antigen (HBsAg). We obtained engineered E. coli expressing humanized anti-HBs Fab fragments by genetic engineering and fermented to express soluble Fab fragments of the antibodies. The anti-HBs activities of Fab purified crystals were quantitatively determined by solid-phase radioimmunoassay (SPRIA) The result is as follows. Materials and Methods I. Preparation of human anti-HBs Fab antibody: The preparation of engineered human anti-HBs Fab fragments was reported in the literature and purified by the same method with anti-HBs positive patient serum.