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在体外实验中发现,单核细胞是血细胞中最易为CMV感染的细胞,在体内,单核细胞感染了CMV后,其提呈抗原的活性降低。本文介绍了在猴模型中,研究单核细胞及病毒抗原载体(Iscom)在CMV感染中的免疫应答。为了避免同种异体抗原的相互作用,作者提纯了猴单核细胞,并将细胞与感染性CMV或高剂量的CMV核衣壳抗原共同孵育后,再注射入猴体内进行免疫。CMV是从骨髓移植患者中分离,在人胚肺细胞内传代1～6次,直出现完全病变时收集细胞,离心分离免疫所需的病毒。CMV核农壳抗原则从感染CMV的人胚肺细胞核内制备。CMV经1%TritonX-100,蔗糖密度梯度离
In vitro experiments found that monocytes are the most easily infected CMV cells in blood cells, in vivo, monocytes infected with CMV, the antigen-presenting activity was reduced. This article describes the study of the immune response of monocytes and viral antigen vectors (Iscom) in CMV infection in a monkey model. To avoid the interaction of alloantigens, the authors purified monkey mononuclear cells and incubated the cells with infectious CMV or high-dose CMV nucleocapsid antigens for subsequent immunization. CMV is isolated from bone marrow transplant patients, passaged in human embryo lung cells 1 to 6 times, until the complete appearance of lesions collected cells, centrifugation of the virus required for immunity. CMV nuclear husk antigen is prepared from human embryonic lung nuclei infected with CMV. CMV via 1% TritonX-100, sucrose density gradient