论文部分内容阅读
I相Q热立克次体Ohio株纯化全细胞抗原(WC)经氯仿-甲醇处理后,获得提取物(CME)和残渣(CMR)。经测定,CMR含有蛋白质、磷酸、中性糖、KDO类似物以及Ⅰ相脂多糖。用CMR作疫苗,腹腔注射C_(57)BL/10 ScN小鼠(内毒素非应答小鼠)进行免疫。注射CMR后的第3天即可在小鼠体内测出抗Ⅱ相特异性抗体,直至35天其效价继续上升;在注射7～14天后出现抗Ⅰ相特异性抗体,随CMR免疫剂量的不同,35天后能否检出抗Ⅰ相抗体不一。免疫小鼠脾脏淋巴细胞在体外对ConA、PHA或PMW等非特异性促有丝分裂原和同源的WC或CMR特
Extract (CME) and Residues (CMR) were obtained after treatment with Chloroform-Methanol (WC) of Phase I heat QI Ohio strain purified whole cell antigen (WC). CMR was determined to contain protein, phosphate, neutral sugars, KDO analogs, and phase I lipopolysaccharide. CMR was used as a vaccine and intraperitoneally injected with C_ (57) BL / 10 ScN mice (endotoxin non-responsive mice) for immunization. Anti-phase II specific antibodies were detected in mice on day 3 after injection of CMR, and their titer continued to increase until 35 days. Anti-phase I specific antibodies appeared after 7 to 14 days of injection, with CMR-immunized Different, 35 days after the detection of anti-Ⅰ phase antibody different. Immune mouse spleen lymphocytes in vitro ConA, PHA or PMW and other non-specific mitogenic and homologous WC or CMR special